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. Author manuscript; available in PMC: 2018 Apr 26.
Published in final edited form as: Cell Syst. 2017 Mar 29;4(4):416–429.e3. doi: 10.1016/j.cels.2017.03.005

Figure 1. A dynamic model of human myeloid differentiation using HL-60s.

Figure 1

(A) HL-60 directed differentiation of neutrophil, monocyte, and macrophage show cell intermediates at varying time-points using Giemsa staining. Intermediate progenitors were characterized based on morphology during the course of differentiation and categorized as: (I) immature macrophage, (II) macrophage < 20μm, (III) macrophage > 20μm, (IV) myelocyte, (V) banded neutrophil, (VI) segmented neutrophil, (VII) monoblast, (VIII) promonocyte, (IX) monocyte. Scale indicates 20μm. (B) Schematic outline of study design. Colored cell identifier denotes myeloid cell-types. (C) Temporal staging of time-points for each cell-type was based on the clustering of RNA-seq and ATAC-seq data. (D) Genome-wide clustering reveals inherent structure of the transcriptome and chromatin landscape during myeloid differentiation (Pearson correlation). Black boxes mark samples grouped as temporal stages. Red boxes indicate LPS induced time-points.