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. 2017 Mar 29;10(2):36. doi: 10.3390/ph10020036

Figure 2.

Figure 2

EC-SEAL variant binding to No Cells, SMCs and ECs (with and without 5 ng/mL TNF-α). (A) No Cells; (B) SMCs; and (C) ECs with and without 5 ng/mL TNF-α were treated with 3 μM of each EC-SEAL variant. Absorbance was measured using a streptavidin-HRP assay to quantify biotinylated peptides on each molecule bound to the surface. None of the EC-SEAL variants displayed a significant difference in binding to unstimulated ECs and ECs stimulated with TNF-α. Variants containing the peptide IEL showed the highest binding affinity to ECs. n = 3; p < 0.05.