Fig. 7. (A) Confocal fluorescence images of HeLa cells treated with 10 μL nanoprobe (OD787nm = 0.5) for 3 h and then NIR irradiation for different times. Green fluorescence at λ ex/em of 488/500–560 nm; red fluorescence at λ ex/em of 633/680–740 nm. Scale bars, 25 μm. (B and C) FL intensity in single cell area obtained from (A) with Leica software. (D and E) In vitro casp-9 and casp-3 kit analysis of cell extracts with the standard curves shown in (F) and (G). (H and I) Calibration curves for activity detection of intracellular casp-9 and casp-3.
