Figure 1.
Alpha diversity (within-sample richness) rarefaction curves based on the observed number of Operational Taxonomic Units, OTUs (image), average Chao1 and Shannon indexes (±SEM) in 24-h in vitro batch culture fermentations inoculated with human feces (n = 3 healthy donors) and administrated with inulin, cellulose, Renetta Canada, Golden Delicious and Pink Lady as the substrates (treatments). Samples were analyzed at 0, 10 and 24 h. Ten sampling repetitions were calculated at an even sampling depth of 11708 sequences. Significant differences (p < 0.05) between treatments at the same time point are indicated with different letters. * Significant differences (p < 0.05) from the 0 h time point within the same treatment.
| Treatment | Chao1 (0 h) | Shannon (0 h) | Chao1 (10 h) | Shannon (10 h) | Chao1 (24 h) | Shannon (24 h) | ||
|---|---|---|---|---|---|---|---|---|
| Inulin | 1076 ± 43 | 6.36 ± 0.36 | 931 ± 91 b,* | 5.32 ± 0.53 b,* | 811 ± 37 b,* | 4.88 ± 0.32 a,* | ||
| Cellulose | 1091 ± 26 | 6.39 ± 0.43 | 1030 ± 66 c,* | 5.99 ± 0.27 c,* | 849 ± 89 c,* | 5.72 ± 0.29 c,* | ||
| Renetta Canada | 1124 ± 84 | 6.49 ± 0.28 | 673 ± 113 a,* | 4.28 ± 0.71 a,* | 774 ± 135 a,* | 4.78 ± 0.63 a,* | ||
| Golden Delicious | 1064 ± 46 | 6.35 ± 0.41 | 724 ± 125 a,* | 4.57 ± 0.64 a,* | 737 ± 31 a,* | 5.20 ± 0.07 b,* | ||
| Pink Lady | 1115 ± 67 | 6.47 ± 0.27 | 748 ± 69 a,* | 4.63 ± 0.66 a,* | 769 ± 48 a,* | 5.14 ± 0.47 b,* |