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. 2017 May 28;9(6):554. doi: 10.3390/nu9060554

Figure 1.

Figure 1

Figure 1

Effects of AWL on lipid accumulation and adipocyte differentiation in 3T3-L1 cells. (A). 3T3-L1 cells were induced to differentiate with DMI and AWL in increasing concentrations (0, 25, and 100 μg/mL) for seven days. The cellular lipid content was assessed by Oil Red O staining. DMI: 0.5 mM 3-IBMX, 100 μM indomethacin, 0.25 μM dexamethasone and 167 nM insulin. AWL: annual wormwood leaves extracts. (B) AWL inhibited TG accumulation in 3T3-L1 adipocytes. Three independent experiments were used to represent the error bars. * p < 0.05, ** p < 0.01. (C) cytotoxicity of AWL in 3T3-L1 cells. Viability of the AWL-treated cells was determined by the MTT assay. The values are presented as the means ± SD. The data shown are representative of at least three independent experiments.