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. 2017 May 28;9(6):554. doi: 10.3390/nu9060554

Figure 2.

Figure 2

Figure 2

Effects of AWL on adipogenic factors in 3T3-L1 adipocytes. (A) AWL decreased the expression of adipogenic factors in 3T3-L1 adipocytes at day 4 by RT-PCR. Similar results were obtained by three independent experiments; (B) AWL inhibited the expression of adipogenic factors in 3T3-L1 adipocytes at day 7 by RT-PCR. The figure is representative of three independent experiments with identical results. Optical density analysis was performed to quantify the levels of mRNA expression with β-actin as loading control. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with DMI group. (C) AWL inhibited the expression of genes related to adipogenesis and lipogenesis in 3T3-L1 adipocytes at days 4 and 7 as determined by a Western blot assay.