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Representative illustration of the experimental design. Forty-eight hours after seeding, HT29 cells were stimulated with 100 U/mL IFNγ as inflammatory stimulus. To investigate the protective effect of curcumin, cells were pre-incubated with 1 µM curcumin (derived from Curcuma Longa) at different time points: 24, 12, 6, 4, 2, or 0 h before the IFNγ treatment. At 96 h of culture (48 h after the addition of IFNγ) cells were harvested for analysis.
