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Melatonin attenuates the activation of ASK1 in insulin stimulated SH-SY5Y cells. Immunofluorescence staining was performed to check the activation of ASK1 (p-ASK1). Confocal microscopy analysis was performed to visualize p-ASK1. SH-SY5Y cells were pretreated with melatonin for 24 h before insulin stimulation. p-ASK1 is represented by red staining, nuclear DNA is indicated by DAPI staining (blue color), and the combined images are presented; Control: non-treated cells, Mel: melatonin.
