Figure 6.

Homology of the putative helicase with RecQ helicases and dh repeats. The 5.6 kb sequence of SPAC212.11 is represented as a rectangle. Horizontal lines above the gene indicate the regions spanned by primers used in this study. P_3' was the fragment of SPAC212.11 on the microarray (180 bases), and P_5' was used in Southern hybridizations (642 bases). Region P_dh was amplified in RT-PCR experiments (Figure 7) to detect dh repeat forward and reverse strands. Solid black rectangles are regions of homology with dh repeats found at centromeres and in the K region of the mating-type locus. The predicted amino-acid sequence of the region marked with cross-hatching has homology with the RecQ helicase family. The BLAST expect (E) value is shown, with the exception that the approximately 70 bp region of homology to dh repeats 3' of the putative RecQ helicase domain has an E value of 2 × 10^-8.