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. 2017 Jun 20;9(6):635. doi: 10.3390/nu9060635

Figure 4.

Figure 4

(A) Gene expression of Wnt1, Wnt3a and Wnt10b in 3T3L1 cells transfected with mmu-miR-27b-3p, mmu-miR-34b-5p or mmu-miR760-5p mimics after 96 h of differentiation. White bars corresponding to Control and grey bars corresponding to 3T3L1 transfected with mmu-miR-27b-3p, mmu-miR-34b-5p or mmu-miR760-5p mimics. * Significative down-regulation was considered when the fold change was ≤−0.5 for microRNA expressions and ≤−0.6 for mimic experiment; (B) Triacylglycerol accumulation in 3T3-L1 cells transfected with mmu-miR-27b-3p, mmu-miR-34b-5p or mmu-miR760-5p mimics according to Oil Red O assay. Control (C+)—cells cultivated with differentiation medium; Guarana (150 µg/mL)—cells cultivated with differentiation medium + guarana (150 µg/mL) during 96 h; 27b-3p, 34b-5p and 760-5p—cells transfected with mmu-miR-27b-3p, mmu-miR-34b-5p or mmu-miR760-5p (respectively) cultivated with differentiation medium. * p < 0.05 and *** p < 0.01 compared to Control; (C) Illustrative images of Oil Red O in 3T3L1 cells Control, Guarana (150 µg/mL) and transfected with mmu-miR-27b-3p, mmu-miR-34b-5p or mmu-miR760-5p mimics (magnification = 200×).