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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2016 Sep 25;101:10–19. doi: 10.1016/j.freeradbiomed.2016.09.021

Fig. 2.

Fig. 2

4-HNE effects on TRPV1 activation and survival. A) Summary of current-voltage (I–V) data using whole cell patch-clamp electrophysiology, examining effects of capsaicin-induced TRPV1 currents with or without 1-hr pre-treatment with 10 μM 4-HNE in transiently transfected HEK cells with WT-rTRPV1 (n=9 cells, capsaicin; n=9 cells, 4-HNE). B) HEK cells were investigated for cell survival in the presence of increasing 4-HNE concentrations for 1-h. HEK cells demonstrated an LC50 of 84.9 μM (R2=0.861) *P < 0.05 vs. WT analyzed by two-way ANOVA (Panel A and B). *P < 0.05 vs. baseline analyzed by two-way ANOVA; #P < 0.05 vs. control analyzed by two-way ANOVA.