Table 1.
Enhancement of tubulin polymerization by different Tat and derived peptides
Compounds | Concentration | ΔOD350 nm | ΔOD ratio |
none | 0.31 | 1 | |
Tat HxB2 | 8 μM | 1.01 | 3.31 |
Tat OYI | 8 μM | 0.80 | 2.63 |
Tat ELI | 8 μM | 1.61 | 5.27 |
Paclitaxel | 15 μM | 0.55 | 1.79 |
Pep38–72 OYI | 8 μM | 0.78 | 2.56 |
Pep38–72 ELI | 8 μM | 1.59 | 5.20 |
Pep73–101 OYI | 8 μM | 0.31 | 1.01 |
Pep73–99 ELI | 8 μM | 0.30 | 1 |
15 μM tubulin is incubated at 37°C in buffer, the increase of ΔOD350 nm is followed and the value of the plateau is indicated in the column ΔOD350 nm. Lane 1 is the control with tubulin alone (none), lanes 2–4 are Tat variants, lane 5 is the paclitaxel control, lanes 6–9 are the Tat peptides. The ΔOD350 nm ratio is the value that the ΔOD350 nm plateau reaches in presence of the indicated product divided by the value of the ΔOD350 nm plateau reached with tubulin alone.