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. 2017 Jun 12;127(7):2751–2764. doi: 10.1172/JCI90921

Figure 4. Treatment with AZ505 delayed cyst growth in conditional Pkd1-knockout mice.

Figure 4

(A) Schedule of the induction and treatment of Pkd1 inducible knockout mice. (B) Histological examination of kidneys from Pkd1fl/fl:tamoxifen-Cre mice injected daily with AZ505 or DMSO vehicle control from P42 to 6 months. Scale bar: 2 mm. (C) Percent cystic area relative to total kidney section area of kidneys from Pkd1fl/fl:tamoxifen-Cre mice treated with AZ505 (n = 14) or DMSO (n = 14). (D and E) Treatment with AZ505 compared with DMSO decreased KW/BW ratios (D) and BUN levels (E) in Pkd1fl/fl:tamoxifen-Cre mice. (F) AZ505 treatment reduced cyst-lining epithelial cell proliferation in kidneys from Pkd1fl/fl:tamoxifen-Cre mice as detected with Ki67 staining. Scale bars: 100 μm. (G) AZ505 treatment induced cyst-lining epithelial cell death in kidneys from Pkd1fl/fl:tamoxifen-Cre mice as detected by TUNEL assay. Scale bars: 100 μm. The images in F and G are also shown in Supplemental Figure 3, C and D.