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. 2017 Jun 19;127(7):2855–2867. doi: 10.1172/JCI89934

Figure 5. Blocking cholesterol biosynthesis protects Chrebp–/– mice from fructose-induced liver injury.

Figure 5

(A) Male 8-week-old Chrebp–/– mice were pretreated by oral gavage with atorvastatin (20 mg/kg BW/day) or vehicle for 2 days, followed by 8 days of 70% HFrD feeding plus atorvastatin or vehicle gavage (n = 6/group). (B and C) Liver total cholesterol and free cholesterol levels were determined. Liver injury was assessed by (D) ALT assay, (E and F) H&E staining, and (G) TUNEL staining (arrowheads indicate apoptotic cells). (H) HMGCR and apoptotic markers were measured by Western blotting. *P < 0.05 and **P < 0.01, by 2-tailed Student’s t test.