Figure 1.
Protective effects of MESA against MPP+-induced toxicity. (A) PC12 cells plated at a density of 1.5 × 105 cells/well and pretreated with MESA. After 2 h incubation, 400 μM of MPP+ was added to the culture medium and incubated for 48 h. MTT assay was performed to determine the cell viability. (B) Worms were treated with 2 mM of MPP+ for 48 h and MESA was added 30 min before MPP+ treatment. Data are expressed as the mean ± S.D. and results are obtained from three independent assays with triplicate determinations. Significance of difference between MESA treatment and MPP+-treated control was determined by a one-way ANOVA, followed by a Tukey mean comparison post hoc test. #p < 0.05 compared with vehicle alone. **p < 0.01 compared with MPP+-treated control.