Figure 5.

Effects of MESA on the lifespan of C. elegans. Worms were grown in the NGM agar plate at 25 °C in the absence or presence of MESA after embryo isolation. The mortality of each group was determined by daily counting of surviving and dead animals. (A) The lifespan of MESA-treated (250 μg/mL) N2 and NL5901 worms was plotted as a survival curve using Kaplan-Meier analysis. (B) The mean lifespan of worms was calculated from the survival curves and data are expressed as the mean ± S.E.M. Statistical difference between the curves was analyzed by the log-rank test. Differences compared to the control were considered significant at ***p < 0.001.