Figure 1.

Workflow for iterative cell enrichment and coexpression analysis to identify PRCE genes in Arabidopsis roots. Genes induced under P starvation (Supplemental Data Set S1A; Misson et al., 2005) were overlaid with data sets describing transcriptional root cell type-specific enrichment (top; Supplemental Data Set S1B; Brady et al., 2007), identifying a subset of 181 genes (middle; Supplemental Data Set S1C). At bottom, putative interactors for these genes were identified using the Arabidopsis Interactions Viewer (Supplemental Data Set S1D; Geisler Lee et al., 2007) and were then overlaid with the cell type-specific enrichment data as described above (Supplemental Data Set S1E). Candidate genes (in red) were selected for detailed molecular and physiological characterization (Supplemental Data Set S1F).