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. 2017 May 17;174(3):1764–1778. doi: 10.1104/pp.16.01898

Figure 1.

Figure 1.

MYB target genes are differentially silenced by miR159. A, Sequence of miR159-binding sites in GAMYB-like and DUO1 transcripts. Nucleotides underlined represent the cleavage site, lower-case blue letters represent mismatches to miR159a, and G:U pairing is shown in uppercase blue letters. B, Different phenotypic categories based on 35S-GAMYB-like expression; none (indistinguishable from wild-type), mild (display of some leaf curl), and severe (two or more leaves showing the abaxial side from an aerial view). Scale bar represents 1 cm. C, Percentage of 35S:MYB and 35S:mMYB primary transformants showing None, Mild, and Severe phenotypes. n = the number of primary transformants. D, Transcript levels of the MYB-downstream gene CP1 in 35S:MYB and 35S:mMYB primary transformants measured by qRT-PCR. RNA was extracted from two independent biological pools of 30 to 50 randomly selected, 15-d-old primary transformants. Col-0 and mir159ab were used as controls, and mRNA levels were normalized to CYCLOPHILIN. The two measurements are shown as dots, with the bar representing the mean. E, Phenotypic characteristics of 40-d-old 35S:DUO1 and 35S:mDUO1 primary transformants. Scale bar represents 1 cm. F, Transcript levels of the DUO1-downstream gene MGH3 in 35S:DUO1 and 35S:mDUO1 primary transformants. Measurements are the average of three technical replicates.