Figure 2.

MYB3 interacts with a small family of four LNK proteins in yeast and in planta. A to C, LNK1, LNK2, LNK3, LNK4, and LNK1 deletion derivatives (LNK1∆N1 and LNK1∆N2) interact with MYB3 and MYB3∆N in yeast. B, Schematic representation of the deletion derivatives of both LNK1 and MYB3. Yeast cells expressing LNK1, LNK2, LNK3, LNK4, LNK1∆N1, LNK1∆N2, and LNK1∆C proteins fused to the GAL4 AD and MYB3, MYB3∆N, and MYB3∆C fused to the GAL4 BD were spotted on SD/-LW medium to select for the plasmids and on SD/-LWH medium containing 10 mm 3-amino-1,2,4-triazole (3AT) to select for transcriptional activation of the His-3 gene. Growth was monitored after 5 d. Yeast cells transformed with empty plasmids pAS2.1 and pACT2, expressing GAL4 BD and AD, respectively, were used as controls in A and C. D, BiFC assays in planta. YFP fluorescence images alone or merged with bright-field images of Arabidopsis cell suspension protoplasts cotransfected with constructs encoding the indicated fusion proteins with YFP at the C terminus or the N terminus. Bar = 20 µm.