Figure 3.

Two conserved Asp residues of LNKs are required for protein interactions. A, Schematic representation of mutation of two conserved residues in either R1 or R2 region of LNK1. B, LNK1D556N, LNK1D614N, and LNK1D556N-D614N interact with MYB3 in quantitative Y2H assays. A liquid culture β-galactosidase assay was performed on the transformed yeasts. The activity of β-galactosidase was measured in arbitrary units. Values are means ± sd of three biological repeats. Asterisks indicate statistically significant differences compared with MYB3, MYB3D556N, and MYB3D614N (P < 0.05, Student’s t test).