ENT domain of LNK1 promotes the activity of MYB3/LNK1 complex-dependent repression of C4H. A, Schematic representation of mutation of two conserved residues in either the R1 or R2 region of LNK3 and chimeric protein of ENT-LNK3. B, Transactivation assays in Arabidopsis protoplasts that were cotransfected with plasmids carrying C4Hpro-GUS and overexpression vectors containing 35S:MYB3, 35S:LNK3, 35S:ENT-LNK3, 35S:ENT-LNK3D192N, 35S:ENT-LNK3D244N, or 35S:ENT-LNK3D192N-D244N, as indicated. Values are means ± sd of three biological repeats. Asterisks indicate statistically significant differences compared with C4Hpro-GUS, 35S:MYB3, 35S:LNK3, or 35S:ENT-LNK3 (P < 0.05, Student’s t test).