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. 2017 May 16;174(3):1897–1912. doi: 10.1104/pp.17.00273

Figure 3.

Figure 3.

Overexpression of miR858 confers enhanced resistance to H. schachtii. A, Constitutive overexpression of miR858 in four independent transgenic Arabidopsis lines resulted in significant decreases in MYB83 expression levels. The expression levels of MYB83 were determined in the roots of 2-week-old transgenic lines relative to the wild-type Col-0 plants using qPCR. Shown are average expression levels obtained from three biological samples ± se. Statistically significant differences were determined using t tests (*P < 0.01). B and C, Root (C) and shoot (B) phenotypes of 3-week-old transgenic Arabidopsis plants overexpressing miR858. D, Nematode infection assays of the miR858 overexpression lines showing reduced susceptibility to H. schachtii compared with the wild-type Col-0 plants. Shown are average numbers of J4 females per root system ± se (n = 20) at 3 weeks postinoculation. Statistically significant differences from wild-type Col-0 plants were determined using t tests (*P < 0.05).