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. 2017 May 16;174(3):1897–1912. doi: 10.1104/pp.17.00273

Figure 8.

Figure 8.

miR858/MYB83 regulatory loop involves MYB12. A and B, MYB83 positively regulates the expression of miR858. The expression levels of primary and mature miR858 transcripts were quantified in the roots of 2-week-old rMYB83 overexpression plants (A) as well as the myb83 mutant line CS1004395 (B) relative to the wild-type Col-0 or Col-4 plants, respectively using qPCR. Shown are relative expression values obtained from three biological samples ± se. Statistically significant differences were determined using t tests (*P < 0.05). C and D, MYB12 phenocopied the effects of MYB83 on plant susceptibility to H. schachtii. Three independent transgenic lines overexpressing the 35S:rMYB12 construct increased susceptibility to H. schachtii compared with the wild-type Col-0 plants (C), whereas the myb12 mutant line FLAG_150B05 showed reduced susceptibility compared with the wild-type Wassilewskija plants (D). Shown are average numbers of J4 females per root system ± se (n = 20) at 3 weeks postinoculation. Statistically significant differences from the corresponding wild-type plants were determined using t tests (*P < 0.05).