Figure 4.

In situ hybridization analysis of genes preferentially expressed at key stages of wheat inflorescence development. In situ localization of homologs of rice OsSPL14 (A–D), OsBBTI (E–H), and OsMADS7 (I–L): DR stage (A, E, and I), FM stage (B, F, and J), AM stage (C, G, and K), and sense probe (D, H, and L). The wheat homolog of OsSPL14 was highly expressed in leaf and bract primordia in the DR stage and decreased with the development of wheat inflorescence (arrows in A–C). The wheat homolog of OsBBTI was highly expressed during FM at the bases of the floret and spikelet meristems (E–H). The expression of the OsMADS7 wheat homolog was initially detected in the floret meristems and later was specifically expressed in the stamen and lodicule primordia during the development of floral organs. BP, Bract primordium; St, stamen; Lo, lodicule. Bars: 200 μm.