Table I. Chlorophyll fluorescence parameters measured in leaves of wild-type, hcf222 mutant, and complemented plants (introduced fusion construct is indicated in subscript).
Mean values for at least three plants are shown. Whole seedlings were measured with the following light intensities: 500-ms saturating light pulses, 5,000 μmol m−2 s−1; and 4 min of actinic light, 120 μmol m−2 s−1. Fv/Fm, Maximum quantum yield of PSII; ΦPSII, effective quantum yield of PSII; qL, fraction of open PSII centers; NPQ, nonphotochemical quenching. Student’s t test significance levels are as follows: *, P < 0.05; **, P < 0.01; and ***, P < 0.001.
| Plant | Fv/Fm | ΦPSII | qL | NPQ |
|---|---|---|---|---|
| Wild-type Col-0 | 0.87 ± 0.02 | 0.68 ± 0.07 | 0.55 ± 0.08 | 0.54 ± 0.2 |
| hcf222-1 | 0.80 ± 0.06 | 0.28 ± 0.15* | 0.11 ± 0,05*** | 0.10 ± 0.02** |
| hcf222-2 | 0.65 ± 0.06** | 0.008 ± 0.01*** | 0.004 ± 0.007*** | 0.008 ± 0.01** |
| Complemented plants | ||||
| hcf222-1cHCF222-HA | 0.86 ± 0.02 | 0.74 ± 0.03 | 0.51 ± 0.08 | 0.34 ± 0.13 |
| hcf222-2cHCF222-HA | 0.85 ± 0.009 | 0.64 ± 0.03 | 0.45 ± 0.1 | 0.30 ± 0.25 |
| hcf222-2cHCF222-GFP | 0.85 ± 0.04 | 0.71 ± 0.06 | 0.42 ± 0.05* | 0.43 ± 0.17 |
| hcf222-2CTP-cHCF222-GFP | 0.86 ± 0.01 | 0.65 ± 0.02 | 0.44 ± 0.01 | 0.30 ± 0.03 |