Table I. Fatty acid content and composition of 1- to 2-DAF and mature green siliques from mutant, complemented, and OE lines of SMO1-1 and ACBP1.
Boldface values indicate statistically significant (P < 0.01) difference from the wild type by Student’s t test: H, higher than wild type; L, lower than wild type. ND, Not detected. Fatty acids were extracted from siliques of 6- to 7-week-old Arabidopsis. Each value represents the mean of four replicates ± sd.
| Genotype | Silique Stage | Total | Relative Composition |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 16:0 | 16:1 | 16:3 | 16:0-OH | 18:0 | 18:1Δ9 | 18:1Δ11 | 18:2 | 18:3 | 20:0 | 20:1Δ11 | 20:1Δ13 | 20:2 | 20:3 | 22:0 | 22:1 | |||
| % dry wt | mol % | |||||||||||||||||
| smo1-1 | 1–2 DAF | 5.06 ± 0.45H | 21.12 ± 0.62 | 1.61 ± 0.04 | 4.63 ± 0.09H | 0.39 ± 0.05 | 2.34 ± 0.10L | 2.69 ± 0.09H | 0.30 ± 0.00 | 27.42 ± 0.49 | 32.62 ± 0.46 | 0.95 ± 0.04 | 2.12 ± 0.70 | ND | 2.88 ± 1.25 | ND | 0.92 ± 0.12 | ND |
| Mature green | 7.50 ± 0.25H | 15.84 ± 0.44 | 0.93 ± 0.05L | 2.39 ± 0.04L | 0.25 ± 0.01 | 3.60 ± 0.11 | 9.47 ± 0.49 | 1.96 ± 0.05 | 30.44 ± 1.42 | 21.14 ± 0.60 | 1.61 ± 0.10 | 6.88 ± 0.63 | 1.63 ± 0.17 | 2.25 ± 0.15 | 0.22 ± 0.07 | 0.85 ± 0.09 | 0.54 ± 0.06 | |
| cSMO1-1 | 1–2 DAF | 4.30 ± 0.13 | 21.40 ± 0.15 | 1.41 ± 0.05 | 3.75 ± 0.12 | 0.47 ± 0.06 | 2.57 ± 0.09 | 2.59 ± 0.02 | 0.28 ± 0.02 | 28.46 ± 0.53 | 29.38 ± 0.23 | 0.92 ± 0.06 | 2.54 ± 0.15 | ND | 5.23 ± 0.80 | ND | 0.99 ± 0.05 | ND |
| Mature green | 6.60 ± 0.13 | 16.74 ± 0.31 | 1.17 ± 0.10 | 2.71 ± 0.09H | 0.25 ± 0.03 | 3.47 ± 0.06 | 9.06 ± 0.40 | 1.96 ± 0.15 | 29.87 ± 0.50 | 21.07 ± 0.32 | 1.56 ± 0.05 | 6.80 ± 0.16 | 1.52 ± 0.18 | 2.14 ± 0.57 | 0.27 ± 0.09 | 0.85 ± 0.13 | 0.56 ± 0.10 | |
| SMO1-1 OE-1 | 1–2 DAF | 4.44 ± 0.18 | 21.32 ± 0.28 | 1.48 ± 0.07 | 4.05 ± 0.21 | 0.33 ± 0.02 | 2.48 ± 0.14 | 2.48 ± 0.03 | 0.32 ± 0.02 | 28.79 ± 1.21 | 31.30 ± 1.16 | 0.98 ± 0.03 | 2.15 ± 0.09 | ND | 3.35 ± 0.64 | ND | 0.98 ± 0.07 | ND |
| Mature green | 6.83 ± 0.13 | 16.17 ± 0.08 | 1.30 ± 0.03H | 2.65 ± 0.08H | 0.24 ± 0.03 | 3.36 ± 0.07L | 8.97 ± 0.34 | 1.92 ± 0.07 | 29.85 ± 0.26 | 22.03 ± 0.13 | 1.59 ± 0.05 | 6.75 ± 0.09 | 1.55 ± 0.02 | 1.95 ± 0.11 | 0.23 ± 0.01 | 0.87 ± 0.03 | 0.57 ± 0.04 | |
| SMO1-1 OE-2 | 1–2 DAF | 4.09 ± 0.17 | 23.12 ± 0.81 | 1.56 ± 0.09 | 3.72 ± 0.18 | 0.34 ± 0.03 | 2.58 ± 0.13 | 2.60 ± 0.12 | 0.32 ± 0.03 | 29.73 ± 0.85 | 29.60 ± 0.47 | 1.00 ± 0.02 | 2.35 ± 0.23 | ND | 2.11 ± 1.43 | ND | 0.98 ± 0.04 | ND |
| Mature green | 6.58 ± 0.54 | 16.20 ± 0.52 | 1.29 ± 0.07H | 2.67 ± 0.05H | 0.28 ± 0.03 | 3.14 ± 0.18L | 9.41 ± 0.20 | 2.00 ± 0.02 | 30.68 ± 0.09 | 20.65 ± 0.22 | 1.58 ± 0.04 | 6.75 ± 0.37 | 1.53 ± 0.07 | 2.11 ± 0.15 | 0.30 ± 0.08 | 0.87 ± 0.04 | 0.57 ± 0.04 | |
| acbp1 | 1–2 DAF | 4.50 ± 0.44 | 20.94 ± 0.52 | 1.43 ± 0.17 | 4.43 ± 0.11H | 0.42 ± 0.10 | 2.42 ± 0.13L | 2.39 ± 0.10 | 0.28 ± 0.03 | 27.38 ± 1.66 | 31.72 ± 1.02 | 0.97 ± 0.07 | 2.49 ± 0.51 | ND | 4.18 ± 1.48 | ND | 0.96 ± 0.09 | ND |
| Mature green | 8.37 ± 0.50H | 14.38 ± 0.12L | 1.24 ± 0.06H | 2.78 ± 0.13H | 0.26 ± 0.01 | 3.55 ± 0.08 | 9.88 ± 0.19 | 1.97 ± 0.12 | 30.66 ± 0.32 | 21.08 ± 0.30 | 1.65 ± 0.11 | 7.25 ± 0.77 | 1.54 ± 0.07 | 1.96 ± 0.27 | 0.31 ± 0.05 | 0.93 ± 0.06 | 0.56 ± 0.11 | |
| cACBP1 | 1–2 DAF | 4.06 ± 0.32 | 21.93 ± 0.58 | 1.42 ± 0.04 | 3.66 ± 0.13 | 0.48 ± 0.12 | 2.80 ± 0.13 | 2.67 ± 0.14 | 0.29 ± 0.02 | 27.42 ± 1.02 | 30.53 ± 1.30 | 1.01 ± 0.06 | 2.13 ± 0.63 | ND | 4.68 ± 1.66 | ND | 0.99 ± 0.11 | ND |
| Mature green | 6.90 ± 0.28 | 16.28 ± 0.69 | 1.21 ± 0.08 | 2.55 ± 0.06 | 0.29 ± 0.02 | 3.90 ± 0.08H | 9.49 ± 0.04 | 1.99 ± 0.05 | 30.39 ± 0.30 | 20.86 ± 0.63 | 1.67 ± 0.04 | 6.31 ± 0.23 | 1.41 ± 0.08 | 2.05 ± 0.35 | 0.24 ± 0.03 | 0.88 ± 0.05 | 0.48 ± 0.03 | |
| ACBP1 OE-1 | 1–2 DAF | 4.12 ± 0.12 | 21.81 ± 0.24 | 1.46 ± 0.17 | 3.85 ± 0.03 | 0.36 ± 0.06 | 2.68 ± 0.03 | 2.69 ± 0.05H | 0.29 ± 0.02 | 28.17 ± 0.46 | 32.07 ± 0.54 | 0.96 ± 0.01 | 2.10 ± 0.39 | ND | 2.63 ± 0.90 | ND | 0.93 ± 0.04 | ND |
| Mature green | 6.58 ± 0.08 | 15.78 ± 0.21 | 1.14 ± 0.04 | 2.34 ± 0.08 | 0.25 ± 0.02 | 3.71 ± 0.03H | 9.17 ± 0.10 | 1.94 ± 0.04 | 30.34 ± 0.54 | 21.59 ± 0.50 | 1.64 ± 0.01 | 6.74 ± 0.13 | 1.40 ± 0.08 | 2.23 ± 0.18 | 0.27 ± 0.04 | 0.93 ± 0.04 | 0.52 ± 0.00 | |
| ACBP1 OE-2 | 1–2 DAF | 3.83 ± 0.30 | 22.25 ± 0.13 | 1.58 ± 0.10 | 4.13 ± 0.04 | 0.38 ± 0.02 | 2.74 ± 0.02 | 2.78 ± 0.07H | 0.31 ± 0.02 | 27.21 ± 0.26 | 31.83 ± 0.40 | 1.04 ± 0.04 | 2.21 ± 0.32 | ND | 2.51 ± 0.24 | ND | 1.02 ± 0.02 | ND |
| Mature green | 6.78 ± 0.06 | 16.09 ± 0.11 | 1.19 ± 0.03 | 2.45 ± 0.18 | 0.29 ± 0.05 | 4.17 ± 0.01H | 9.33 ± 0.18 | 1.95 ± 0.06 | 30.08 ± 0.62 | 21.40 ± 0.55 | 1.58 ± 0.04 | 6.37 ± 0.17 | 1.50 ± 0.23 | 2.05 ± 0.12 | 0.20 ± 0.05 | 0.79 ± 0.04 | 0.56 ± 0.03 | |
| ACBP1+/−smo1-1 | 1–2 DAF | 4.78 ± 0.27H | 22.00 ± 0.85 | 1.30 ± 0.13 | 4.45 ± 0.06H | 0.32 ± 0.04 | 2.10 ± 0.12L | 2.35 ± 0.15 | 0.30 ± 0.02 | 27.49 ± 0.62 | 32.03 ± 0.37 | 1.02 ± 0.10 | 2.48 ± 0.25 | ND | 3.15 ± 1.07 | ND | 1.01 ± 0.01 | ND |
| Mature green | 7.34 ± 0.06H | 15.10 ± 0.40L | 1.14 ± 0.15 | 2.46 ± 0.17 | 0.26 ± 0.03 | 3.54 ± 0.04 | 9.45 ± 0.05 | 1.91 ± 0.09 | 30.45 ± 0.27 | 21.87 ± 0.26 | 1.66 ± 0.13 | 6.82 ± 0.85 | 1.57 ± 0.07 | 2.02 ± 0.13 | 0.25 ± 0.06 | 0.89 ± 0.03 | 0.61 ± 0.08 | |
| Wild type | 1–2 DAF | 3.97 ± 0.32 | 21.65 ± 0.36 | 1.44 ± 0.10 | 3.86 ± 0.26 | 0.48 ± 0.13 | 2.80 ± 0.10 | 2.42 ± 0.09 | 0.27 ± 0.02 | 27.94 ± 0.57 | 29.73 ± 1.58 | 1.02 ± 0.10 | 2.45 ± 0.66 | ND | 4.94 ± 1.89 | ND | 0.99 ± 0.04 | ND |
| Mature green | 6.75 ± 0.26 | 16.46 ± 0.38 | 1.08 ± 0.06 | 2.48 ± 0.02 | 0.27 ± 0.02 | 3.52 ± 0.04 | 9.39 ± 0.28 | 1.95 ± 0.04 | 30.18 ± 0.40 | 21.38 ± 0.85 | 1.58 ± 0.04 | 6.48 ± 0.34 | 1.47 ± 0.06 | 2.15 ± 0.44 | 0.23 ± 0.02 | 0.86 ± 0.05 | 0.51 ± 0.05 | |