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. 2017 May 9;174(3):1576–1594. doi: 10.1104/pp.17.00466

Figure 7.

Figure 7.

HA:AtPRA1.F4 overexpression differentially inhibits the trafficking of TGN-localized SNARE proteins at the Golgi apparatus. A to C, Effects of HA:AtPRA1.F4 overexpression on the localization of SYP41:GFP, SYP51:GFP, and SYP61:GFP. The indicated SNARE and KAM1ΔC:mRFP constructs were transformed into wild-type protoplasts together with HA:AtPRA1.F4 or R6 (empty vector), and the localization of SYP41:GFP (A), SYP51:GFP (B), or SYP61:GFP (C) was examined. For colocalization of SNAREs with KAM1ΔC:mRFP, images (n = 35 and 30 for SYP41, n = 35 and 45 for SYP51, and n = 21 and 30 for SYP61 without and with HA:AtPRA1.F4, respectively) were analyzed using the Pearson and Spearman correlation coefficient colocalization plug-in of ImageJ software. The results are shown as scatterplots (right-most images). Bars = 10 μm. D, Quantification of colocalization. The extent of colocalization of the indicated SNARE proteins with KAM1ΔC:mRFP was calculated and presented in relative values. Error bars represent sd (n = 37 and 53 for SYP41, n = 34 and 41 for SYP51, and n = 19 and 20 for SYP61 without and with HA:AtPRA1.F4, respectively). Asterisks indicate statistically significant differences at P ≤ 0.05 (*) or P ≤ 0.001 (***) between R6 and HA:AtPRA1.F4. TE, Transient expression in protoplasts.