Figure 3.

Structural organization of the CaMYB31 gene and phylogenetic relationship of CaMYB31 with other plant MYB transcription factors. A, Schematic diagram of CaMYB31 showing the coding regions (black boxes), 5′ and 3′ UTRs (gray boxes), introns (solid lines), R2R3-MYB domains (dotted lines), and the fragment sequence of CaMYB31 used for virus-induced gene silencing (VIGS). B, Phylogenetic tree constructed using the neighbor-joining method based on the MYB domain alignment using MEGA6 software. The bootstrap values are shown as percentages (1,000 replicates) when greater than 50%. The clades were grouped as reported previously for Arabidopsis (Stracke et al., 2001; highlighted with colored diamonds for each subgroup), while CaMYB31 (highlighted with blue letters) grouped with solanaceous sequences (marked with red circles). Accession numbers for all protein sequences are listed in Supplemental Table S2.