Figure 2.

Phenotype of an At-psa3 mutant. A, Diagram of At-PSA3 (AT3G55250) showing the position of the T-DNA insertion in the At-psa3 allele analyzed here. The nucleotide position of the insertion with respect to the start codon is indicated in parentheses. B, Phenotype of At-psa3 mutants and transgene complementation of the high chlorophyll fluorescence (hcf) phenotype. Plants were grown on MS-agar medium for 3 weeks and were then rearranged for photography. The hcf phenotype of the same plants is shown below. The plant labeled At-psa3 comp is a homozygous mutant harboring a transgene encoding At-PSA3 with a C-terminal 3×FLAG tag. Bar = 1 cm. C, Immunoblot analysis of subunits of photosynthetic complexes in At-psa3 mutants. Total protein isolated from seedling leaves was loaded on an equal protein basis, as illustrated in the excerpt of the Ponceau-stained blot shown below. A dilution series of the wild-type (WT) sample was included to estimate relative protein levels. Replicate blots were probed with antibodies to the indicated proteins.