Figure 6.

Tissue-specific and Na-responsive expression of OsMGT1. Ato E, Expression of OsMGT1 in different root region. Immunostaining with an anti-GFP antibody was performed in different root zone of pOsMGT1-GFP transgenic rice (A­–D) and wild-type rice (E), including longitudinal section of root (A), cross sections at 5 mm (B and E), and 30 mm (C and D) from root apex. F to K, Expression of OsMGT1 expression to salt stress. Immunostaining of root segments (20 mm from root tips; F and G), basal node (H), leaf sheath (I and J), and leaf blade (K), after exposure to 0 (F) or 50 mm (G–K) NaCl for 24 h. Red color shows signal from GFP antibody detected with a secondary antibody. Cyan color shows cell wall autofluorescence. Yellow-dotted areas in C and I were magnified in D and J, respectively. EN, Endodermis; EX, exodermis. Five independent transgenic lines were investigated, and the representative results are shown. Bars = 500 μm (A) and 100 μm (B–K).