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. 2017 May 12;174(3):1747–1763. doi: 10.1104/pp.17.00542

Figure 4.

Figure 4.

OsSGR and OsNYC3 are the direct target genes of OsNAC2. A, Interaction of OsNAC2 with the promoters of OsSGR and OsNYC3 by yeast one-hybrid assays. The yeast cells were grown on SD/-Leu/-Trp/-His/+30 mm 3-amino-1, 2,4-triazole medium. B, Interaction of OsNAC2 with the promoters of OsSGR and OsNYC3 by dual-luciferase promoter activation assays in tobacco. C and D, ChIP-qPCR assays. Total protein extracted from 35S:OsNAC2-mGFP transgenic plants hydroponically cultivated for 4 weeks were immunoprecipitated with an anti-GFP antibody. Fragmented genomic DNA was eluted from the protein-DNA complexes and subjected to qPCR analysis. The long black bars represent promoter regions for which we designed primers. The numbers under the bar show the distance from ATG start codon. Short bars represents for the corresponding region of each pair of primers on the promoter. Error bars are the se for three biological repeats: *P < 0.05 and **P < 0.01.