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. 2017 May 12;174(3):1747–1763. doi: 10.1104/pp.17.00542

Figure 7.

Figure 7.

Interactions between OsNAC2 and the promoters of OsNCED3, OsZEP1, and OsABA8ox1. A, Interaction of OsNAC2 with the promoters of OsNCED3, OsZEP1, and OsABA8ox1 by yeast one-hybrid assays. The yeast cells were grown on SD/-Leu/-Trp/-His/+30 mm 3-amino-1, 2,4-triazole medium. B to D, ChIP-qPCR assays of OsNAC2 binding to the promoters of OsNCED3 (B), OsZEP1 (C), and OsABA8ox1 (D). Total protein extracted from 35S:OsNAC2-mGFP transgenic plants hydroponically cultivated for 4 weeks was immunoprecipitated with an anti-GFP antibody. Fragmented genomic DNA was eluted from the protein-DNA complexes and subjected to qPCR analysis. The long black bars represent for promoter region for which we designed primers. The numbers under the bar show the distance from ATG start codon. Short bars represents for the corresponding region of each pair of primers on the promoter. Error bars are the se for three biological repeats: *P < 0.05, **P < 0.01, and ***P < 0.001.