Figure 1.

Biochemical properties of Rubisco activase isoforms from rice and agave. A, SDS-PAGE analysis of purified activases and rice Rubisco. A total of 4 µg of protein loaded per lane. B, Rubisco activation assays of RuBP-inhibited rice Rubisco (ER) performed at 25°C (0.5 µm Rubisco active sites, 5 µm activase protomer). C, ATPase activity assays (5 µm protomer, 25°C) and quantified Rubisco activase activity of the different activase isoforms. Error bars show mean and sd of independent experiments with n as indicated. Significant differences (P < 0.05) are denoted by different letters at the base of each bar.