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. 2017 May 30;174(3):1609–1620. doi: 10.1104/pp.17.00584

Figure 3.

Figure 3.

The tonoplast targeting of PAT10 is mediated by AP-3. A and B, CLSM of root epidermal cells from PAT10g-GFP pat4-2 transgenic plants upon 1 min of FM4-64 staining (A) or pulse labeled with FM4-64 and followed by BFA treatment for 50 min (B). C, CLSM of root epidermal cells from ProUBQ10:GFP-INT1;pat4-2 transgenic plants upon 1 min of FM4-64 staining. D to G, CLSM of root epidermal cells from PAT10g-GFP;ProUBQ10:RFP-SUC4;pat4-2 (D), PAT10g-GFP;WAVE22R;pat4-2 (E), PAT10g-GFP;VHA-a1-RFP;pat4-2 (F), and PAT10g-GFP;ProUBQ10:RFP-VSR2;pat4-2 (G) transgenic plants. The Pearson correlation coefficient (Rp) and the Spearman correlation coefficient (Rs) are indicated on the scatterplots at the right side of each micrograph. Bars = 10 µm.