Figure 7.

The HOPS complex is required for AP-3-mediated vacuolar trafficking. A, CLSM of a wild-type (WT) or vps41-1 pollen tube coexpressing PAT10g-GFP and CBL2-RFP. Merges of the GFP and RFP channels are shown at the bottom. B and C, CLSM of a wild-type or vps41-1 pollen tube expressing GFP-VAMP711 (B) or GFP-INT1 (C). Merges of the GFP and transmission channels are shown at the bottom. Dotted lines illustrate the silhouettes of pollen tubes. D, CLSM of a wild-type pollen tube expressing WAVE9R (red) and stained with OG (green). E and F, CLSM of a wild-type (E) or vps41-1 pollen tube (F) stained with OG. Three-dimensional surface rendering images showing vacuolar structures are at the bottom. Bars = 10 µm.