Figure 8.

Cartoon model showing the AP-3-dependent PAT10 trafficking. A, In the wild type (WT), PAT10 was selectively packed into the Sar-1c-dependent COPII complex at the ER and destined for the Golgi. AP-3 mediates the transport of PAT10 from the Golgi to the vacuoles together with the HOPS complex, where cytosolic CBL2 is S-acylated and anchored to the tonoplast. B, In the mutants of AP-3, PAT10 was retained at the Golgi, where cytosolic CBL2 is S-acylated and anchored to the Golgi. Due to active vesicular trafficking at the Golgi, S-acylated CBL2 follows Rab5-dependent trafficking from the Golgi to the TGN/EE, where it targets to the PM or the tonoplast nonselectively.