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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Nature. 2017 Mar 15;543(7646):573–576. doi: 10.1038/nature21671

Figure 2. METTL3/14 and FTO oppositely regulate m6A RNA at DNA damage sites.

Figure 2

a, Control or METTL3 (M3 KD) U2OS cells were microirradiated, incubated at 37°C for 2 min, and stained as indicated. Arrows: representative γH2A.X-positive damage sites with METTL3 colocalization. b, WT or METTL3 KO U2OS cells expressing or not WT (WT-R) or catalytically inactive (Cat-R) METTL3, treated as in (a). c, WT or FTO KO U2OS cells subjected or not (0′) to 50 J UVC, treated as in (a). The percentage of γH2A.X-positive damage sites displaying colocalizing METTL3 (a), m6A (b), or relative m6A intensity (c), are indicated. n=3, 50 cells per replicate. Scale bar, 20 μm.