Skip to main content
. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Nature. 2017 Mar 15;543(7646):573–576. doi: 10.1038/nature21671

Figure 3. METTL3 is important for UV-induced DNA damage repair and cell survival.

Figure 3

a, Dot-blot detection of CPDs in genomic DNA of WT or METTL3 KO (M3 KO) U2OS cells, subjected or not (0 h) to 15 J UVC. Loading control: Methylene blue. b, Nascent RNA in cells from (a) expressing or not WT (WT-R) or catalytically inactive (Cat-R) METTL3, subjected or not (0 h) to 25 J UVC and incubated at 37°C as indicated. (a–b) n=3, 50 cells per replicate. Scale bar, 20 μm. c, Quantification of (b) with each time-point normalized to its unirradiated control. d, Normalized colony formation of UVC-irradiated cell lines from (b). n≥3, results shown as mean ± SEM. (**) P ≤ 0.01.