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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Free Radic Biol Med. 2017 Feb 22;106:288–301. doi: 10.1016/j.freeradbiomed.2017.02.041

Figure 1. Wogonin (WG) inhibited the IL1-β induced inflammatory mediators in human OA chondrocytes.

Figure 1

Primary human OA chondrocytes were pre-treated with Wogonin (10–50 μM) for 2 h followed by treatment with IL-1β (1 ng/ml) for 16 h. At the end of treatment culture supernatant were collected and chondrocytes were harvested. Cell lysate were prepared using RIPA buffer for immunoblot analysis or RNA were isolated for real time PCR analysis. (A) Expression of IL-6, COX-2 and iNOS was measured by quantitative PCR using the TaqMan assay system (Life Technologies). β-actin was used as endogenous expression control. (B) Protein expression of secreted IL-6, cytosolic IL-6, COX-2 and iNOS was investigated by immunoblotting using antibodies against indicated protein. β-actin was used as a control for equal loading. Specific signal intensities were quantified by ImageJ software. (C–D) Secreted levels of IL-6 (C) and PGE2 production (D) were measured in the culture supernatant by ELISA (E) Production of NO was estimated in supernatant using Griess assay as described in methods. Bar graph represents mean±SD from two subjects. *p≤0.01, as compared to control, #≤0.01, as compared to IL-1β.