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. 2017 Jun 29;12(6):e0179937. doi: 10.1371/journal.pone.0179937

Fig 5. The detection times of the Vch-UPT-LF assay under pre-incubation conditions.

Fig 5

The shortest incubation time, when the T/C ratios of the up-converting phosphor technology-based lateral flow assay for simultaneous detection of O1 and O139 (Vch-UPT-LF) were higher than the cutoff value, was defined as the detection time (*). (A) The detection times of channel 1 of Vch-UPT-LF for detecting V. cholerea O1. (B) The detection times of channel 2 of Vch-UPT-LF for detecting V. cholerae O139. The detection times were all less than 7 h for 101 ~ 104 CFU mL−1 of V. cholerae O1 and O139, while they could reach 4 h for both O1 and O139 at 104 CFU mL−1.