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. 2017 Jun 29;12(6):e0180155. doi: 10.1371/journal.pone.0180155

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© 2017 Fernández-Niño et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Model of the modular network containing two units: the repressilator [6] (blue triangle), a network where three genes (tetR, lacI, λcl) suppress the expression of each other, and the artificial cell-cell communication module (red square). This artificial module was built from components of the quorum sensing systems of Vibrio fisheri and Agrobacterium tumefaciens. The LuxI protein synthesizes an Auto-inductor AI (3-oxo-C6HL). Upon reaching a critical concentration, AI is bound by the LuxR receptor; AI-LuxR binds to the luxbox promoter region and activates gene transcription. In the artificial cell-cell communication module, the AI-LuxR complex stimulates production of GFP (Green Fluorescent Protein), expression of the lactonase AiiB, and LacI, the repressor of pLac01. AiiB can hydrolyze AI molecules creating a negative feedback to restart the system. (B) Scheme of the recombinant plasmid coding for the artificial cell-cell communication module.