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. 2017 Jul 1;28(13):1754–1767. doi: 10.1091/mbc.E16-12-0862

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© 2017 Sarker et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — NHE3 activity of Caco-2 cells expressing NHE3-S719D is inhibited by forskolin, carbachol, and wortmannin, but TBB has no effect. (A) Caco-2/bbe cells were infected with Ad-HA-NHE3-S719D at 12 d postconfluency and activity measured ∼2 d later. Effects of wortmannin (100 nM, 30 min), forskolin (25 μM, 30 min), and carbachol (25 μM, 5 min) were determined. All three inhibited NHE3 activity similarly, and the magnitude of the inhibition was similar to that in NHE3-WT (Figures 2 and 4). (B) The effect of TBB (30 μM, 30 min) on NHE3 in Caco-2/bbe cells expressing NHE3-S719D was not significant. n, number of experiments. p values compared by paired t tests and ANOVA.