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Glycerol biosynthesis contributes to deactivation of Ssp2. Western blot and quantification showing activation kinetics of Ssp2-pT189 in wild-type and gpd1Δ gpd2Δ cells in response to 1 M KCl osmotic stress. We used α-myc as loading control for total Ssp2. Values displayed are the mean ± SD based on three individual biological replicates. An unpaired t test was performed for statistical analyses, and p values were based on two-tailed distributions (**p < 0.01).
