Table 1. List of 40 influenza strains and strength of the signal measured when probed with pan-HA mAb cocktail.
| Strain |
Type of antigen | Production platform | Strength of the signal1 |
|---|---|---|---|
| H1N1/A/Puerto Rico/8/1934 | rHA Virus Virus Virus |
Insect cells Eggs MDCK cells HEK293 cells |
+++ +++ ++ ++ |
| H1N1/A/California/07/2009 | rHA Virus Virus Virus Virus VLP |
HEK293 cells Eggs MDCK cells HEK293 cells Avian cells Plant |
++ +++ ++ ++ + +++ |
| H1N1/A/Wilson Smith/33 | Virus | HEK293 cells | ++ |
| H1N1 Avian like Swine | Virus | MDCK cells | +++ |
| H1N2 Reassortant human like A/Scotland/410440/94 | Virus | MDCK cells | +++ |
| H2N2 A/Singapore/1/57 | Virus | Eggs | ++ |
| H3N1/A/Victoria/361/2011 | VLP | Plant | +++ |
| H3N2/A/Wisconsin/67/2005 | rHA | Insect cells | - |
| H3N2 A/Aichi/2/1968 | Virus | HEK293 cells | + |
| H3N2 A/Hong Kong/8/1968 | Virus | HEK293 cells | + |
| H3N2/A/Brisbane/10/2007 | rHA | Insect cells | +++ |
| H3N2 A/New York/55/01 | Virus | Eggs | +++ |
| H3N2 A/Texas/50/2012 | Virus | HEK293 cells | + |
| H3N2A/Panama/2007/99 | Virus | Eggs | +++ |
| H4N6 A/Duck/Czechoslovakia/56 | Virus | Eggs | ++ |
| H5N1/A/Vietnam/1203/2004 | rHA | Insect cells | +++ |
| H5N1/A/Indonesia/5/2005 | rHA VLP |
Insect cells Plant |
+++ +++ |
| H5N2 A/Finch/England | Virus | MDCK cells | +++ |
| H5N9 A/Turkey/Wisconsin/68 | Virus | Eggs | ++ |
| H6N2 A/Turkey/Massachusetts/3740/65 | Virus | Eggs | ++ |
| H7N7/A/Netherlands/219/2003 | rHA | Insect cells | +++ |
| H7N7 A/Equine/Prague/1/56 | Virus | Eggs | +++ |
| H7N9 A/Anhui/1/2013 | rHA Virus |
Insect cells Eggs |
++ + |
| H7N9 A/Shanghai/2/2013 | rHA | HEK293 cells | ++ |
| H7N9/A/Pigeon/Shanghai/S1069/2013 | rHA | HEK293 cells | ++ |
| H7N3/A/Turkey/Oregon/71 | Virus | Eggs | +++ |
| H7N1 A/Turkey/Italy/977/1999 | Virus | MDCK cells | + |
| H8N4 A/Turkey/Ontario/6118/68 | Virus | Eggs | +++ |
| H9N2/A/Hong Kong/1073/1999 | rHA | Insect cells | ++ |
| H9N2 A/Turkey/Wisconsin/1/66 | Virus | Eggs | +++ |
| H10N8 A/Quail/Italy/1117/65 | Virus | Eggs | +++ |
| H11N6 A/Duck/England/56 | Virus | Eggs | +++ |
| H12N6 A/Duck/Wisconsin/480/79 | Virus | Eggs | +++ |
| H12N5 A/Duck/Alberta/60/76 | Virus | MDCK cells | +++ |
| H13N6 A/Gull/Maryland/704/77 | Virus | Eggs | ++ |
| B/Malaysia/2506/2004 | Virus | Eggs | +++ |
| B/Brisbane/60/2008 | rHA | Insect cells | +++ |
| B/Lee/40 | Virus | HEK293 cells | ++ |
| B/Massachusetts/2/2012 |
Virus Virus Virus |
Avian cells MDCK cells HEK293 cells |
- +++ +++ |
| B/Florida/04/2006 |
Virus Virus |
MDCK cells Eggs |
+++ +++ |
1The signal was measured by immunoblotting in Relative Light Units (RLU):–means no signal (<200 RLU), + means low signal (500 to 2000RLU), ++ means medium signal (2000 to 15000 RLU), +++ means strong signal (15000 to 40000 RLU). Negative controls were under 200 RLU.