Table 2. Validation of selected RNA-Seq results by qPCR.

Locus IDa	J2315 orthologsb	Descriptiona	Gene name	Log2 FC MT vs WTc	Log2 FC MT vs WTd
I35_0767	BCAL3106	Urease alpha subunit	ureC	-5.0	-5.2
I35_2151	BCAL2223	Glutamine synthetase	glnA	-3.6	-4.8
I35_4771	BCAM0858	Polysaccharide export lipoprotein	bceE	-1.2	-2.6
I35_4928	BCAM1009	O-antigen acetylase		-1.1	-4
I35_6233	BCAM2340	3-(3-hydroxyalkanoyloxy) alkanoic acids synthase	rhlA	-1.6	-2.9
I35_0133	BCAL0126	Flagellar motor rotation protein	motA	-0.9	-2.7
I35_3089	BCAL0577	Flagellar hook-associated protein	flgL	-2.4	-2.9
I35_3103	BCAL0561	Flagellar biosynthesis protein	flgN	-1.8	-3.3

^aNomenclature and description according to GenBank file (accession no. HG938370, HG938371, and HG938372).

^bOrthologs were identified as described in the Material and Methods section.

^cFold change (FC) of transcription determined by qPCR, comparing the ntrC mutant (MT) with the wild type (WT) grown in AB minimal medium with a shift experiment. The standard deviation is less than 10% of the fold change.

^dFold change (FC) of transcription determined by RNA-Seq, comparing the ntrC mutant (MT) with the wild type (WT) grown in AB minimal medium (shift experiment).