Fig 1. Sensitivity of E2348/69, LRT9 and their rpoS∷Tn10 mutants to environmental stresses.

Overnight cultures of E2348/69, LRT9 and their respective rpoS∷Tn10 mutants were resuspended in (A) 0.9% NaCl supplemented with 6 mM H₂O₂ (oxidative stress); (B) 2 M NaCl (osmotic stress); (C) EG buffer pH 2 (acid stress). Samples were harvested at the indicated time points and plated on LB-agar for CFU counting. (♦) LRT9; (▲) E2348/69; (▶) LRT9 rpoS∷Tn10; (◼) E2348/69 rpoS∷Tn10. Each point corresponds to the mean ± SEM of three independent experiments.