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. 2017 Jun 29;12(6):e0180381. doi: 10.1371/journal.pone.0180381

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© 2017 Mata et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Competition for adherence to epithelial cells between the wild-type strains (E2348/69 and LRT9) against their respective rpoS∷Tn10 mutants (A) and between E2348/69 and LRT9 strains against each other (B). Bacteria were suspended in DMEM over a monolayer of HEp-2 cells for 3 hours. The adhered bacteria were then released and seeded on L-agar or on L-agar supplemented with tetracycline for CFU counting.