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. 2017 Jun 29;12(6):e0180381. doi: 10.1371/journal.pone.0180381

Table 1. Bacterial strains and plasmids used in this study.

Strains Genotype Source
E2348/69 EPEC 0127:H6 NalR [27]
CFP1 E2348/69 rpoS∷Tn10 This study
LRT9 EPEC O111:abH2 [28]
GMF237 LRT9 rpoS∷Tn10 This study
LG01 LRT9 lacZ∷Tn5 Lab collection
MC4100 F- araD139 (argF-lac)U169 rpsL150 deoC1 relA1 thiA ptsF25 flbB5301 rbsR [29]
BS878 MC4100 rpoS∷Tn10 This study
BS1230 LRT9 bfpA∷SPA-Km [30]
BS1332 GMF237 bfpA∷SPA-Km rpoS∷Tn10 This study
BS1298 LRT9 eae∷SPA-Km [30]
BS1307 GMF237 eae∷SPA-Km rpoS∷Tn10 This study
Plasmids Relevant feature Source
pRKlacZ 290 Low copy vector carrying a promoterless lacZ [31]
pGM30 bfpA promoter cloned upstream of lacZ in plasmid pRKlacZ 290-SpR This study
pGM36 tir-eae promoter cloned upstream of lacZ in plasmid pRKlacZ 290-SpR This study
pNP5 rpoS+ cloned in the low-copy plasmid vector pACT3 [32]