Table 1. Bacterial strains and plasmids used in this study.
Strains | Genotype | Source |
E2348/69 | EPEC 0127:H6 NalR | [27] |
CFP1 | E2348/69 rpoS∷Tn10 | This study |
LRT9 | EPEC O111:abH2 | [28] |
GMF237 | LRT9 rpoS∷Tn10 | This study |
LG01 | LRT9 lacZ∷Tn5 | Lab collection |
MC4100 | F- araD139 (argF-lac)U169 rpsL150 deoC1 relA1 thiA ptsF25 flbB5301 rbsR | [29] |
BS878 | MC4100 rpoS∷Tn10 | This study |
BS1230 | LRT9 bfpA∷SPA-Km | [30] |
BS1332 | GMF237 bfpA∷SPA-Km rpoS∷Tn10 | This study |
BS1298 | LRT9 eae∷SPA-Km | [30] |
BS1307 | GMF237 eae∷SPA-Km rpoS∷Tn10 | This study |
Plasmids | Relevant feature | Source |
pRKlacZ 290 | Low copy vector carrying a promoterless lacZ | [31] |
pGM30 | bfpA promoter cloned upstream of lacZ in plasmid pRKlacZ 290-SpR | This study |
pGM36 | tir-eae promoter cloned upstream of lacZ in plasmid pRKlacZ 290-SpR | This study |
pNP5 | rpoS+ cloned in the low-copy plasmid vector pACT3 | [32] |