Figure 3. Plasmids are lost in a cas9-dependent manner in the absence of selection.

Fraction of cells containing pKHS67 was determined by CFU-cam^R/Total CFU. Cultures were passaged for 2 weeks in the absence of chloramphenicol. The geometric mean and geometric standard deviation from two independent biological replicates each from two transconjugants (total 4 unique transconjugants) are shown. Strains are defined in Figure 3.

DOI: http://dx.doi.org/10.7554/eLife.26664.010