Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 23;13:1633–1645. doi: 10.2147/NDT.S129081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Bu et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

ASD induced upregulation of intracellular ROS and mitochondrial ROS generation and apoptosis of LCLs.

Notes: (A) The representative images showed changes in mitochondrial transmembrane potential (ΔΨ_m) in the control and ASD LCLs. Magnification, 100×. The graph represents the ratio of red/green fluorescence (n=3). (B) The representative images showed intracellular ROS detected by DCFH-DA staining. Magnification, 100×. The graph represents the intensity of green fluorescence (n=3). (C) The representative images showed mitochondria ROS production detected by MitoSOX staining. Magnification, 100×. The MitoSOX fluorescence intensity in the control and ASD LCLs (n=3). (D) Cells were double stained with Annexin V-FITC and PI and analyzed by flow cytometry. And percentage of apoptotic cells is shown in the histogram (n=3). (E) Enzymatic activity of mitochondrial complex I and complex III in the control and ASD LCLs (n=3). *P<0.05 compared with the control LCLs.

Abbreviations: ASD, autistic spectrum disorder; LCL, lymphoblastoid cell line; ROS, reactive oxygen species.

ASD induced upregulation of intracellular ROS and mitochondrial ROS generation and apoptosis of LCLs.

Notes: (A) The representative images showed changes in mitochondrial transmembrane potential (ΔΨ_m) in the control and ASD LCLs. Magnification, 100×. The graph represents the ratio of red/green fluorescence (n=3). (B) The representative images showed intracellular ROS detected by DCFH-DA staining. Magnification, 100×. The graph represents the intensity of green fluorescence (n=3). (C) The representative images showed mitochondria ROS production detected by MitoSOX staining. Magnification, 100×. The MitoSOX fluorescence intensity in the control and ASD LCLs (n=3). (D) Cells were double stained with Annexin V-FITC and PI and analyzed by flow cytometry. And percentage of apoptotic cells is shown in the histogram (n=3). (E) Enzymatic activity of mitochondrial complex I and complex III in the control and ASD LCLs (n=3). *P<0.05 compared with the control LCLs.

Abbreviations: ASD, autistic spectrum disorder; LCL, lymphoblastoid cell line; ROS, reactive oxygen species.